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Fluorescein Free Acid
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Description
Fluorescein Free Acid
Other names: fluorescein, fluorescein free acid, Acid Yellow 73, CI 45350, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
CAS number: 2321-07-5
EC number: 219-031-8
Molecular formula: C20H12O5
Molar mass: 332.31 g/mol
Appearance: orange to red or reddish-brown powder or crystals
Fluorescein free acid is an organic xanthene dye with intense fluorescent properties. The product is used as a dye, fluorescent marker and reagent in laboratory, analytical, research and technical applications. It exhibits characteristic fluorescence, making it suitable for studies requiring visualisation, labelling or fluorescence detection.
Chemical and physical properties:
Form: powder or crystals
Colour: orange to red or reddish-brown
Melting point: approximately 320°C
Solubility in water: limited
Solubility in methanol: approximately 0.1 g/10 ml, orange to intensely orange solution
Absorption maximum λmax: approximately 486–501 nm, depending on measurement conditions
Fluorescence: λex approximately 490 nm, λem approximately 514 nm in 0.1 M Tris pH 8.0
Applications:
Fluorescent dye:
Used as a dye and fluorescent marker in laboratory work and analytical studies.
Analytical chemistry:
Used as an auxiliary reagent in methods requiring fluorescence detection, labelling or tracing the presence of substances.
Material and biological research:
May be used as a fluorescent probe and reference material in studies on fluorescent systems.
Technical applications:
Used in colouring formulations, visualisation tests, flow tracing and work requiring an intense fluorescent dye.
Key properties:
High fluorescence: enables the product to be used as a fluorescent marker and probe.
Xanthene structure: responsible for the characteristic optical and colour properties of the compound.
Intense colour: the product usually occurs as an orange to red or reddish-brown powder.
Analytical application: suitable for research, laboratory and technical work requiring fluorescent dyes.
Storage:
Store the product in a tightly closed container in a dry and well-ventilated place.
Store at room temperature.
Protect from moisture, contamination and direct sunlight.
Keep away from strong oxidising agents.
Handling:
Avoid the formation and inhalation of dust.
Avoid contact with eyes.
During handling, use appropriate personal protective equipment, including protective gloves, eye protection and a dust mask under dusty conditions.
Wash hands thoroughly after handling.