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Nile Blue
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Description
Nile Blue
Other Names: Nile Blue A, C.I. 51180, Oxazine 811
CAS Number: 3625-57-8
Sum Formula: C₄₀H₄₀N₆O₆S
Molar Mass: 732.80 g/mol
Appearance: Dark green to blue crystalline powder
Nile Blue is a synthetic oxazine dye known for its strong fluorescent properties and affinity for biological structures. It is primarily used in analytical and materials sciences for imaging, fluorescence microscopy, and redox chemistry.
Chemical and Physical Properties
Density: Approx. 1.3 g/cm³ (estimated)
Melting Point: > 300°C (decomposes)
Boiling Point: Not applicable (decomposes)
Solubility in Water: Slightly soluble
Solubility in Other Solvents: Soluble in ethanol and methanol; insoluble in nonpolar solvents
Odor: Odorless
Applications
Fluorescent Dye: Used in microscopy and imaging of biological samples due to its red emission under blue light excitation.
Redox Chemistry: Acts as a redox indicator in electrochemical and enzymatic assays.
Biological Staining: Employed in histological and cytological staining protocols.
Photophysical Research: Studied in materials chemistry for its photostability and spectral characteristics.
Safety
This chemical does not meet GHS hazard criteria for 100% (5 of 5) of all reports.
Handling and Storage
Storage Conditions: Store in a tightly sealed container in a cool, dry, and well-ventilated place. Protect from light and moisture.
Handling Precautions: Avoid formation of dust. Handle with appropriate protective equipment and follow good laboratory safety practices.