Evans Blue

Evans Blue
Other Names: Direct Blue 53, T-1824, Diazo Blue, C.I. 23860

CAS Number: 314-13-6
Sum Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Molar Mass: 960.81 g/mol
Appearance: Dark blue to black powder

Evans Blue is a synthetic azo dye from the direct dye class. It is a tetrasodium salt of a complex diazo compound, characterized by its high affinity for serum albumin. It is widely used in research and industrial settings, particularly in studies of vascular permeability, dye tracing, and material staining.

Chemical and Physical Properties
Density: Approx. 1.8 g/cm³ (estimated)
Melting Point: > 300°C (decomposes)
Boiling Point: Not applicable (decomposes)
Solubility in Water: Freely soluble
Solubility in Other Solvents: Slightly soluble in ethanol; insoluble in nonpolar solvents
Odor: Odorless

Applications
Vascular and Permeability Studies: Used in experimental research to evaluate blood-brain barrier integrity and tissue perfusion.
Tracer Dye: Applied in hydrological tracing and leak detection systems.
Staining Agent: Utilized for coloring and marking in biological and industrial samples.
Spectroscopy and Dye Chemistry: Employed as a model azo dye in the study of dye behavior and molecular interactions.

Safety

Signal Word: Warning

Handling and Storage
Storage Conditions: Store in a tightly closed container in a cool, dry, and well-ventilated area. Protect from light and moisture.
Handling Precautions: Avoid dust formation. Handle with appropriate protective equipment and in accordance with good industrial hygiene and safety practices.