Eosin Dye Cell Staining

Eosin Y Dye

Other names: Eosin Y, Acid Red 87, bromoeosin, 2′,4′,5′,7′-tetrabromofluorescein, eosin yellowish, Solvent Red 43, CI 45380:2

CAS Number: 15086-94-9

EC Number: 239-138-3

Sum Formula: C20H8Br4O5

Molar Mass: 647.89 g/mol

Appearance: red to red-pink crystalline powder

Eosin Y is a synthetic xanthene dye with fluorescent properties. It is widely used in laboratory, analytical and microscopy applications, especially as a staining reagent and contrast dye. In biological and histological procedures, eosin Y is commonly used as a counterstain to highlight selected cellular and tissue structures.

The product may be used in analytical chemistry, microscopy, staining procedures and laboratory research involving dye-based visualization and contrast methods.

Chemical and Physical Properties:

Form: powder

Color: red to red-pink

Solubility in Water: limited, depending on product form and working conditions

Solubility in Ethanol: soluble, approximately 1 mg/ml according to Sigma-Aldrich product data

Type of Compound: xanthene dye, brominated fluorescein derivative

Fluorescence: fluorescent dye

Colour Index: CI 45380:2

Applications:

Analytical Chemistry:

Used as a dye and counterstain in analytical and laboratory staining procedures.

Microscopy:

Applied in microscopy to provide visual contrast for selected structures during light-microscopy observation.

Histology and Cytology:

Used in staining procedures where red or pink coloration is required to contrast with other staining components.

Laboratory Procedures:

Used as a research dye in dye-binding, visualization and analytical applications.

Key Properties:

Xanthene Dye Structure: belongs to the xanthene dye family.

Fluorescent Character: suitable for applications requiring fluorescent or strongly colored staining behavior.

Intense Color: provides red to pink coloration in staining and visualization procedures.

Laboratory Use: suitable for analytical, microscopy and research applications.

Storage:

Store in a tightly closed container in a dry, cool and well-ventilated place.

Store at room temperature unless otherwise specified by the supplier.

Protect from moisture, contamination and direct sunlight.

Keep away from strong oxidizing agents and incompatible materials.

Handling:

Avoid formation and inhalation of dust.

Use in a well-ventilated area.

Avoid contact with skin and eyes.

Use appropriate personal protective equipment, including protective gloves, eye protection and a dust mask when dust may be generated.

This product is intended strictly for technical, laboratory and research use. It is not intended for consumption, medical, pharmaceutical, cosmetic or feed applications.