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Opis
Toluidine Blue
Other Names: Toluidine Blue O, (7-amino-8-methylphenothiazin-3-ylidene)dimethylammonium chloride, Basic Blue 17
CAS Number: 92-31-9
Sum Formula: C₁₅H₁₆ClN₃S
Molar Mass: 305.82 g/mol (270.37 g/mol for the cation only — full salt includes Cl⁻ counterion)
Appearance: Dark green to blue crystalline powder
Toluidine Blue is a basic thiazine dye structurally related to methylene blue. It is primarily used in analytical chemistry and microscopy due to its metachromatic staining properties. It binds nucleic acids and acidic tissue components with high affinity.
Chemical and Physical Properties
Density: Approx. 1.2 g/cm³ (estimated)
Melting Point: Decomposes above 220°C
Boiling Point: Not applicable (decomposes)
Solubility in Water: Highly soluble
Solubility in Other Solvents: Slightly soluble in alcohol; insoluble in nonpolar solvents
Odor: Odorless
Applications
Biological Staining: Used in histology and cytology for metachromatic staining of acidic tissue components such as mast cell granules and cartilage.
Analytical Chemistry: Acts as an indicator and reagent for nucleic acid detection.
Photodynamic Research: Investigated for its interaction with light and potential in redox or photosensitizing reactions.
Microbiology: Applied in staining of bacteria and fungal elements.
Safety
Signal Word: Warning
Hazard Statements:
H302: Harmful if swallowed.
H315: Causes skin irritation.
H319: Causes serious eye irritation.
H335: May cause respiratory irritation.
Precautionary Statements:
P261: Avoid breathing dust.
P264: Wash hands thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear protective gloves and eye protection.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
Handling and Storage
Storage Conditions: Store in a tightly closed container in a cool, dry, and well-ventilated area. Protect from light and humidity.
Handling Precautions: Avoid dust formation and contact with skin or eyes. Use appropriate personal protective equipment and standard laboratory hygiene protocols.