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Błękit bromofenolowy
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Bromophenol Blue
Other Names: 3',3'',5',5''-Tetrabromophenolsulfonphthalein, Tetrabromophenol sulfonphthalein
CAS Number: 115-39-9
Sum Formula: C₁₉H₁₀Br₄O₅S
Molar Mass: 669.96 g/mol
Appearance: Purple to reddish-brown crystalline powder
Bromophenol blue is a pH indicator dye and acid-base sensitive compound used primarily in analytical chemistry. It is a sulfonephthalein dye that exhibits a distinct color change depending on pH, transitioning from yellow to blue in acidic to basic conditions, respectively.
Chemical and Physical Properties
Density: Approx. 1.9 g/cm³ (estimated)
Melting Point: 273–280°C (decomposes)
Boiling Point: Not applicable (decomposes)
Solubility in Water: Slightly soluble (better in alkaline solution)
Solubility in Other Solvents: Soluble in alcohol and other polar solvents
Odor: Odorless
Applications
pH Indicator: Commonly used in laboratories to monitor pH changes, especially in electrophoresis and titration.
Electrophoresis: Serves as a tracking dye in gel electrophoresis protocols.
Analytical Chemistry: Used in colorimetric assays and visual pH estimation.
Chemical Demonstrations: Employed in educational settings to illustrate acid-base transitions.
Safety
Signal Word: Warning
Hazard Statements:
H315: Causes skin irritation.
H319: Causes serious eye irritation.
H335: May cause respiratory irritation.
Precautionary Statements:
P261: Avoid breathing dust.
P264: Wash hands thoroughly after handling.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear protective gloves, protective clothing, and eye protection.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
P337+P313: If eye irritation persists: Get medical advice/attention.
Handling and Storage
Storage Conditions: Store in a tightly sealed container in a dry, cool, and well-ventilated location. Protect from light and moisture.
Handling Precautions: Avoid formation of dust and contact with skin or eyes. Use personal protective equipment and work in a well-ventilated area.